Do you do have a protocol or instructed antibodies to conduct ICC staining on my fully differentiated ALI cultures?
Most effective outcomes are acquired when restricting extended cell publicity to ambient temperature circumstances. Take into account keeping unused cells in a very humidified incubator with five% CO2 at 37°C when doing bigger experiments.
New to STEMCELL? Sign-up for an account to speedily and easily buy goods online and for one particular-click on access to all educational written content. Develop an Account
Cannot locate the answer you are looking for? Attain out to us and among our pulmonary professionals will get again to you personally.
The STEMdiff™ system provides a standardized method for differentiating hPSCs into epithelial cells which might be later on cultured in 2nd or 3D formats depending on the study wants.
and its subsidiaries and affiliates (“STEMCELL”) to gather and use your information and facts, and ship you newsletters and e-mail in accordance with our privacy plan. Make sure you Call us with any queries that you could have. You'll be able to unsubscribe or change your e-mail Choices Anytime.
Assess the results of take a look at compounds on hematopoietic stem cells with phenotypic and purposeful assessments.
Differentiation into adipocytes, osteoblasts, or chondrocytes with suitable MesenCult™ differentiation systems
Indeed, you’ll find the actions to perform an ICC staining on the epithelial cells cultured for the ALI In this particular protocol. Here is an index of antibodies that could be utilized for the characterization of airway cultures:
To modify your searching cart, make sure you take out The present quote get prior to making modifications towards your cart. In case you need variations on the quote, you Stem cell should Get in touch with your neighborhood revenue agent. Cancel
When designing guide RNAs, it is usually recommended to 지방흡입 pick sequences that align Together with the transcription commence website from the gene of curiosity in which possible.
Complete cell counts working with an automatic cell counter. Note: Reduced viability could be optimized with the duration of collagenase or ACCUTASE™ incubation. It can 지방흡입 be optimized by reducing the quantity of triturations.
Thanks on your fascination In this particular solution. Make sure you supply us together with your Get in touch with info and your neighborhood agent will Get hold of you by using a custom-made quote. In which ideal, they might also aid you using a(n): Estimated delivery time to your region Product sample or special offer you In-lab demonstration By submitting 줄기세포 지방이식 this manner, you will be offering your consent to STEMCELL Technologies copyright Inc.
To change your purchasing cart, make sure you eliminate the current quotation get 지방이식 prior to making alterations in your cart. Should you need changes into the quotation, remember to Make contact with your local revenue agent. Terminate